Isolation of Active Mitochondria From Tomato Fruit
نویسندگان
چکیده
منابع مشابه
Isolation of active mitochondria from tomato fruit.
An improved method for isolating mitochondria from tomato fruit (Lycopersicon esculentum Mill.) is described. The fruit is chilled, and the tissue of the fruit wall cut by hand into very thin slices with a razor blade while immersed in a buffer containing 0.4 m sucrose, 2 mm MgCl(2), 8 mm EDTA, 4 mm cysteine, 10 mm KCl, 0.5 mg per ml bovine serum albumin 50 mm tris-HCl, pH 7.6. The pH is monito...
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It has long been realised that the study of isolated protoplasts offers a potentially new approach to problems of plant cell biology. For this potential to be fully realised, intact protoplasts must be produced, free from contamination by cell debris, on a scale sufficiently large to enable biochemical and physiological experiments to be performed. This aim, though not impossible as has been su...
متن کاملIsolation and sequencing of tomato fruit sucrose synthase cDNA.
Suc synthase (EC 2. 4. 1. 13) catalyzes the reversible reaction: Suc + UDP c-, UDP-Glc + Fru. In plants, its main physiological function is to cleave Suc and generate UDPGlc, which is used in such diverse pathways as starch synthesis, cell wall component synthesis, and energy generation (Huber and Akazawa, 1986; Sung et al., 1988). It has been demonstrated that Suc synthase is a key enzyme duri...
متن کاملIsolation and Identification of Ripening-Related Tomato Fruit Carboxypeptidase.
Tomato (Lycopersicon esculentum) fruit carboxypeptidase active on N-carbobenzoxy Z-L-phenylalanine-L-alanine was found to constitute a family of isoforms whose abundance changed differentially during ripening. A specific polyclonal antibody against the fruit carboxypeptidase was raised in rabbits and used to purify and identify the protein. The data from immunoaffinity chromatography, immunoinh...
متن کاملActivity and functional interaction of alternative oxidase and uncoupling protein in mitochondria from tomato fruit.
Cyanide-resistant alternative oxidase (AOX) is not limited to plant mitochondria and is widespread among several types of protists. The uncoupling protein (UCP) is much more widespread than previously believed, not only in tissues of higher animals but also in plants and in an amoeboid protozoan. The redox energy-dissipating pathway (AOX) and the proton electrochemical gradient energy-dissipati...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1968
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.43.6.883